Ro 25-6981 is primarily used in neuroscience research as a selective antagonist for NMDA receptors, specifically targeting the NR2B subunit. Its application is significant in studying the role of these receptors in various neurological processes and disorders. Researchers utilize Ro 25-6981 to investigate mechanisms underlying conditions such as chronic pain, depression, and neurodegenerative diseases like Alzheimer's. By blocking NR2B-containing NMDA receptors, it helps elucidate their involvement in synaptic plasticity, memory formation, and neuronal excitotoxicity. Additionally, it is employed in experimental models to explore potential therapeutic strategies targeting NMDA receptor dysfunction. This compound has become a valuable tool in understanding the complex signaling pathways in the central nervous system.