WAY-359473 is primarily used in scientific research to study the glycine site of the N-methyl-D-aspartate (NMDA) receptor in the central nervous system. It acts as a potent and selective antagonist at the glycine-binding site on the NMDA receptor complex, which plays a key role in synaptic plasticity, learning, and memory. Due to its selectivity, it is a valuable tool for investigating the physiological and pathological roles of NMDA receptor activation, particularly in conditions such as excitotoxicity, neuropathic pain, and neurodegenerative disorders. It has also been used in preclinical studies to explore potential therapeutic strategies for stroke and other neurological conditions involving glutamate dysregulation. Its application remains confined to laboratory and experimental settings, with no current clinical use in humans.