HEGA-9 (n-Nonyl β-D-glucopyranoside) is a non-ionic detergent widely used in biochemistry and molecular biology for solubilizing membrane proteins. It effectively extracts integral membrane proteins while preserving their native structure and biological activity. HEGA-9 is commonly employed in protein purification workflows, gel electrophoresis (including native PAGE), chromatography, and membrane protein crystallization. Its mild properties make it ideal for applications requiring functional protein studies.