ML335 is primarily used in research settings to study and modulate the activity of Nrf2, a transcription factor involved in the regulation of antioxidant proteins. It is utilized to investigate cellular responses to oxidative stress and to explore potential therapeutic strategies for diseases where oxidative damage plays a key role, such as neurodegenerative disorders, cardiovascular diseases, and cancer. Researchers apply ML335 to inhibit Nrf2 activity, allowing them to better understand its role in cellular defense mechanisms and its impact on disease progression. This compound is particularly valuable in experimental models aimed at identifying novel drug targets and developing treatments that can effectively manage oxidative stress-related conditions.