Used extensively in molecular biology for the isolation of RNA and DNA. It effectively denatures proteins, ensuring the integrity of nucleic acids during extraction. Commonly employed in the preparation of samples for PCR, qPCR, and other downstream applications. Its chaotropic properties disrupt hydrogen bonding, making it ideal for breaking down cell structures and inactivating nucleases. Also utilized in the purification of plasmids and viral RNA, providing high yields and purity. Often found in commercial RNA extraction kits due to its efficiency and reliability.