Used primarily in molecular biology for the isolation of RNA from cells and tissues. It effectively denatures proteins, ensuring the inactivation of RNases that could degrade RNA during extraction. Commonly applied in protocols involving guanidinium thiocyanate-phenol-chloroform extraction, such as the TRIzol method, to obtain high-quality RNA for downstream applications like RT-PCR, qPCR, and RNA sequencing. Its ability to maintain RNA integrity makes it essential for sensitive experiments requiring pure and intact RNA samples.