DFHBI is widely used in biochemical research as a fluorogenic dye, particularly in the study of RNA aptamers. It binds specifically to the Spinach RNA aptamer, enabling real-time visualization and tracking of RNA molecules in live cells. This application is crucial for understanding RNA dynamics, localization, and function within cellular environments. Additionally, DFHBI is utilized in the development of biosensors and imaging tools, aiding in the detection of specific RNA sequences and the monitoring of gene expression. Its ability to emit fluorescence upon binding makes it a valuable tool for advanced molecular biology and diagnostic research.