Digoxigenin monodigitoxoside is primarily used in molecular biology as a labeling agent for nucleic acid probes. It is conjugated with nucleotides to create probes for in situ hybridization, allowing for the detection of specific DNA or RNA sequences in cells or tissues. This compound is also employed in immunohistochemistry, where it serves as a hapten for antibody-based detection systems. Its high specificity and sensitivity make it valuable in diagnostic applications, such as identifying genetic disorders or pathogens. Additionally, it is utilized in Western blotting and ELISA techniques for protein detection and quantification.