This chemical is widely used in biological research for staining DNA in fluorescence microscopy and flow cytometry. It binds specifically to the minor groove of double-stranded DNA, making it a valuable tool for visualizing and quantifying DNA in cells. Researchers often use it to study cell cycle dynamics, apoptosis, and chromatin organization. Its fluorescence properties allow for clear differentiation between live and dead cells, aiding in viability assays. Additionally, it is employed in molecular biology techniques to detect DNA in gels and other experimental setups. Its specificity and stability make it a reliable choice for various nucleic acid staining applications.