Photobiotin acetate salt is widely used in molecular biology for labeling nucleic acids, such as DNA and RNA, to create non-radioactive probes. It is particularly valuable in techniques like Southern blotting, Northern blotting, and in situ hybridization, where it allows for the detection of specific sequences with high sensitivity. The compound is activated by light, enabling it to bind covalently to nucleic acids, making the labeling process efficient and straightforward. Additionally, it is used in biotinylation of proteins and other biomolecules, facilitating their purification or detection through biotin-streptavidin interactions. Its applications extend to research in genomics, proteomics, and diagnostics, where precise and reliable labeling is essential.