Hoechst 33342 analog is widely used in biological research for staining DNA in live and fixed cells. Its ability to bind specifically to the minor groove of double-stranded DNA makes it a valuable tool in fluorescence microscopy and flow cytometry. Researchers utilize it to study cell cycle dynamics, chromatin organization, and nuclear morphology. It is particularly useful in identifying and sorting cells based on DNA content, aiding in cancer research and stem cell studies. Additionally, its permeability to live cells allows for real-time monitoring of cellular processes without causing significant toxicity.