Used as a fluorescent probe in biochemical and cellular imaging due to its strong fluorescence properties. The aminopentylamino side chain allows conjugation to proteins, antibodies, or other biomolecules for specific labeling. It is employed to study nucleic acid structures, protein distributions, and biomolecular interactions in solution and cells. Commonly utilized in fluorescence microscopy, flow cytometry, and spectroscopy to label and track biomolecules in live and fixed cells. Also applied in the development of sensors for detecting specific interactions. Its hydrochloride salt form enhances solubility in aqueous buffers, making it suitable for biological assays with high sensitivity for low-concentration detection.