Ampliflu Red is primarily used as a fluorescent dye in molecular biology applications, especially in nucleic acid detection. It is commonly employed in gel electrophoresis to visualize DNA or RNA after separation, offering a safer alternative to traditional dyes like ethidium bromide. The dye binds to nucleic acids and emits strong fluorescence under UV or blue light, enabling clear imaging of DNA bands. It is compatible with both agarose and polyacrylamide gels and is often used in PCR product analysis, restriction digestion checks, and general nucleic acid quantification. Its high sensitivity allows detection of low-concentration samples, making it suitable for routine lab use in research and diagnostic settings.