Ethidium bromide solution
for fluorescence, ~1% in H 2 O
Reagent
Code: #98592
Alias
Ethidium bromide;-3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, ethidium bromide; EB
CAS Number
1239-45-8
blur_circular Chemical Specifications
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Molecular Information
Weight
394.31 g/mol
Formula
C₂₁H₂₀BrN₃
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Registry Numbers
MDL Number
MFCD00011724
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Physical Properties
Melting Point
260-262 °C (dec.)(lit.)
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Storage & Handling
Storage
2-8°C, away from light
description Product Description
Ethidium bromide solution is widely used in molecular biology for staining nucleic acids, particularly DNA and RNA, in agarose gel electrophoresis. It intercalates between the base pairs of DNA, allowing visualization under ultraviolet light. This application is crucial for analyzing DNA fragments, verifying PCR products, and assessing DNA integrity. Additionally, it is employed in fluorescence microscopy to study chromosome structures and in research involving nucleic acid quantification. However, due to its mutagenic properties, handling requires strict safety precautions, including the use of gloves and proper disposal methods.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Concentration | 0.9-1.1% |
| Appearance | Dark red liquid |
| Infrared Spectrum | Conforms to Structure |
| Max Water | 480 nm |
| pH | 4.2-4.6 |
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