Lambda phage DNA, non-methylated, is widely used in molecular biology research for various applications. It serves as a template for DNA sequencing, allowing researchers to study genetic sequences and mutations. Its non-methylated state makes it ideal for restriction enzyme digestion, as methylation can interfere with enzyme activity. This DNA is also employed in cloning experiments, where it acts as a vector to insert and replicate foreign genes in host organisms. Additionally, it is used in studies of DNA-protein interactions, such as those involving transcription factors and DNA-binding proteins, due to its well-characterized structure. Lambda phage DNA is also a valuable tool in teaching laboratories for demonstrating techniques like PCR, gel electrophoresis, and DNA fingerprinting. Its use in research and education underscores its importance in advancing genetic and biotechnological studies.