6-Azauracil is primarily used in biochemical research as an inhibitor of orotidine monophosphate decarboxylase, an enzyme involved in the biosynthesis of pyrimidine nucleotides. This property makes it valuable for studying nucleic acid metabolism and for investigating the mechanisms of nucleotide synthesis in cells. Additionally, it has been explored for its potential antiviral and anticancer properties due to its ability to interfere with RNA and DNA synthesis. In agricultural research, 6-Azauracil is sometimes used to study plant growth and development, particularly in relation to nucleotide metabolism. Its role in these areas highlights its importance as a tool for understanding fundamental biological processes and developing therapeutic strategies.