6-Chlorouracil is primarily used in biochemical research as a uracil analog to study nucleic acid metabolism and enzymatic processes. It serves as a competitive inhibitor of thymidylate synthase, an enzyme crucial for DNA synthesis, making it valuable in understanding cell proliferation and cancer mechanisms. Additionally, it is utilized in the development of antiviral and anticancer agents due to its ability to interfere with RNA and DNA synthesis. In agricultural research, 6-Chlorouracil is explored for its potential to inhibit plant growth, aiding in the study of herbicides and plant physiology. Its role in these applications highlights its significance in both medical and agricultural sciences.