6-Methyladenine is primarily used in biochemical research as a modified nucleobase. It plays a significant role in studying DNA methylation processes, which are crucial for understanding gene expression regulation. Researchers utilize it to investigate how methylation affects DNA-protein interactions and cellular functions. Additionally, it serves as a valuable tool in epigenetics research to explore the impact of methylation on genetic inheritance and disease development. In molecular biology, it is employed in synthetic oligonucleotide synthesis to create modified DNA strands for various experimental purposes. Its applications extend to the development of diagnostic assays and therapeutic strategies targeting methylation-related disorders.