N4-Benzoylcytosine is primarily utilized in the field of nucleic acid research and organic synthesis. It serves as a protected form of cytosine, a fundamental nucleobase in DNA and RNA. This compound is often employed in the synthesis of oligonucleotides, where it helps in the controlled assembly of nucleotide sequences by protecting the amino group of cytosine during chemical reactions. Additionally, it finds application in the development of modified nucleosides and nucleotides, which are crucial for studying DNA and RNA structure, function, and interactions. Its use extends to the preparation of labeled compounds for biochemical assays and as a building block in the creation of novel therapeutic agents targeting genetic disorders.