This compound is primarily used in the synthesis of oligonucleotides, particularly in the field of molecular biology and genetic research. It serves as a protected nucleoside, where the 4,4′-dimethoxytrityl (DMT) group protects the 5′-hydroxyl during chemical synthesis, ensuring selective reactivity. The iodine atom at the 5-position of cytosine allows for further functionalization or cross-coupling reactions, making it valuable for creating modified DNA or RNA sequences. Its applications extend to the development of probes, primers, and therapeutic oligonucleotides, aiding in studies related to gene expression, diagnostics, and targeted drug delivery. The compound’s stability and reactivity make it a critical building block in solid-phase oligonucleotide synthesis.