This chemical is primarily used in the field of molecular biology and genetic research. It serves as a modified nucleoside, enabling the incorporation of specific functional groups into DNA strands. Its application is crucial in the synthesis of oligonucleotides, where it acts as a building block for creating labeled or modified DNA sequences. The acrylamido group allows for further functionalization, making it valuable in the development of DNA probes and biosensors. Additionally, it is utilized in studies involving DNA-protein interactions, fluorescence labeling, and other advanced molecular techniques. Its unique structure supports the creation of stable and specific DNA conjugates, aiding in diagnostic and therapeutic research.