8-Bromo-cAMP is widely used in cellular and molecular biology research as a cell-permeable analog of cyclic adenosine monophosphate (cAMP). It activates protein kinase A (PKA) and other cAMP-dependent signaling pathways more effectively than natural cAMP due to its resistance to degradation by phosphodiesterases. This makes it a valuable tool for studying intracellular signaling mechanisms related to metabolism, gene expression, cell proliferation, and differentiation. It is commonly applied in studies involving neuronal function, where sustained PKA activation influences synaptic plasticity and memory formation. In endocrinology, 8-Bromo-cAMP is used to mimic hormone actions that rely on cAMP as a second messenger, such as those of glucagon and adrenaline. It also helps stimulate insulin secretion in pancreatic beta-cell research. In cardiovascular research, this compound is used to investigate the regulation of heart rate and contractility mediated through cAMP pathways. Additionally, it has been employed in studies of ion channel regulation and fluid secretion in epithelial cells. Due to its stability and potency, 8-Bromo-cAMP is preferred in long-term experiments requiring persistent elevation of intracellular cAMP levels.