Used in DNA sequencing and PCR amplification to reduce secondary structure formation in GC-rich templates. The modified base enhances polymerase processivity and improves read accuracy in regions prone to hairpin formation. Commonly applied in Sanger sequencing and real-time PCR, especially when amplifying difficult templates. Also used in next-generation sequencing workflows where stable incorporation and reduced bias are required. Suitable for enzymatic synthesis of labeled DNA probes and in applications requiring high fidelity base pairing with improved thermal stability.