It is primarily used in biochemical research as a tool to study nucleic acid metabolism and enzyme mechanisms. This compound acts as a chain terminator in DNA synthesis due to its azido group, making it valuable in sequencing technologies and the study of DNA polymerases. It is also utilized in the development of antiviral drugs, particularly in targeting viral replication processes. Additionally, its incorporation into RNA allows for the investigation of RNA structure and function, aiding in the understanding of gene expression and regulation. Its unique properties make it a useful probe in molecular biology and medicinal chemistry studies.