N6-Ethyladenosine is primarily utilized in research settings, particularly in the study of RNA modifications and their biological roles. It serves as a modified nucleoside that helps scientists understand the impact of ethylation on RNA structure, function, and stability. This compound is also employed in the development of nucleotide analogs for potential therapeutic applications, such as antiviral or anticancer agents. Additionally, it is used in biochemical assays to investigate enzyme activity related to RNA processing and modification pathways. Its role in mimicking natural RNA modifications makes it valuable for studying cellular mechanisms and designing targeted therapies.