2-Chloroinosine is primarily used in biochemical research as a modified nucleoside. It serves as a key intermediate in the synthesis of various nucleoside analogs, which are crucial for studying nucleic acid structure and function. This compound is also employed in the development of antiviral and anticancer agents, as it can be incorporated into RNA to alter its properties and inhibit viral replication or cancer cell growth. Additionally, 2-Chloroinosine is utilized in enzymatic studies to investigate the mechanisms of nucleoside-metabolizing enzymes, providing insights into potential therapeutic targets. Its role in probing RNA interactions and modifications further aids in advancing RNA-based therapeutics and diagnostics.