2'-Amino-2'-deoxyuridine is primarily used in biochemical and medical research as a nucleoside analog. It is incorporated into DNA during replication, making it a valuable tool for studying DNA synthesis and repair mechanisms. Researchers utilize it to investigate the effects of modified nucleosides on cellular processes, particularly in understanding mutagenesis and the behavior of DNA polymerases. Additionally, it has potential applications in antiviral and anticancer research, where its ability to disrupt normal DNA function can be exploited to inhibit the proliferation of viruses or cancer cells. Its role in labeling and tracking DNA in experimental studies also aids in advancing genetic and molecular biology research.