Benzylacyclouridine is primarily utilized in biochemical research as a potent inhibitor of thymidine phosphorylase. This enzyme is involved in the metabolism of thymidine, and its inhibition is crucial in studies focused on understanding and controlling cellular processes related to DNA synthesis and repair. Researchers employ Benzylacyclouridine to investigate the pathways of nucleoside metabolism and to explore its potential therapeutic applications in cancer treatment, where inhibiting thymidine phosphorylase can help in reducing tumor growth and angiogenesis. Additionally, it serves as a valuable tool in the development of antiviral drugs, particularly in targeting viral replication mechanisms that rely on nucleoside analogs. Its role in these areas underscores its importance in advancing medical and pharmacological research.