Used in the solid-phase synthesis of modified oligonucleotides, particularly in antisense therapy and RNA interference (RNAi) applications. The 2'-fluoro modification enhances nuclease resistance and improves binding affinity to complementary RNA strands. The 5-methyluridine group further increases thermal stability of duplexes. The DMT (dimethoxytrityl) group at the 5'-end enables controlled stepwise coupling during synthesis and provides protection for the hydroxyl group. The CE phosphoramidite functionality allows efficient incorporation into DNA or RNA strands via automated synthesizers. This compound is especially valuable in the development of therapeutic oligonucleotides, including siRNA, antisense oligonucleotides, and aptamers, where stability, specificity, and pharmacokinetic properties are critical.