Used in solid-phase oligonucleotide synthesis, this phosphoramidite is essential for incorporating 2′-O-alkyl modifications into RNA strands. The 2′-O-TOM (trimethylsilyl-1-oxyethyl) protecting group enhances RNA stability and nuclease resistance, making it valuable in developing therapeutic oligonucleotides such as antisense drugs, siRNA, and aptamers. Its compatibility with automated synthesizers allows efficient, high-yield RNA strand assembly. After synthesis, the TOM group can be removed under mild acidic conditions, preserving the integrity of the RNA chain. This modification improves pharmacokinetic properties, supporting use in gene silencing and molecular diagnostics.