Used in solid-phase oligonucleotide synthesis to introduce a biotin label through a phosphoramidite group. The DMT-Biotin-C6 moiety allows for efficient coupling to the growing DNA chain, enabling the attachment of biotin at specific positions during synthesis. After deprotection, the biotin tag can be used for affinity capture, such as streptavidin-based purification or detection in hybridization assays. The C6 spacer provides a hexyl linker that reduces steric hindrance, improving binding efficiency to streptavidin. Commonly applied in diagnostic probes, gene synthesis, and molecular biology research where labeled nucleic acids are required for detection or pull-down experiments.