Used in solid-phase oligonucleotide synthesis as a phosphoramidite building block for introducing modified nucleosides into DNA or RNA strands. The compound enables site-specific incorporation of a pyrimidine derivative with a benzamido group, useful in antisense technology, diagnostic probes, and therapeutic oligonucleotides. The protecting groups ensure compatibility with automated synthesis, while the diisopropylphosphoramidite moiety allows for efficient coupling. The 2-cyanoethyl group facilitates later deprotection under mild basic conditions. Commonly applied in research settings for developing nucleic acid-based drugs and functional genomics tools.