Used in solid-phase oligonucleotide synthesis as a phosphoramidite building block for incorporating modified nucleosides into DNA strands. The benzamido and cyanoethoxy groups provide protection during synthesis, ensuring selective coupling and high yield. The diisopropylphosphoramidite moiety enables efficient phosphite bond formation with the growing oligonucleotide chain on a solid support. After chain assembly, the protecting groups are removed under standard deprotection conditions, yielding functionalized oligonucleotides used in antisense therapy, PCR, and gene editing applications. Its stereochemistry ensures proper sugar conformation, enhancing hybridization specificity and nuclease resistance in the final product.