Used in oligonucleotide synthesis, 6-VIC Phosphoramidite enables the incorporation of a visible light-excitable fluorophore into DNA or RNA strands. It is commonly applied in the preparation of fluorescently labeled probes for real-time PCR, hybridization assays, and molecular beacon technologies. The dye’s strong emission in the visible range allows for sensitive detection and quantification of nucleic acids. Its phosphoramidite group ensures efficient coupling during solid-phase synthesis, making it suitable for automated DNA synthesizers. Widely used in genomics, diagnostics, and gene expression studies where fluorescent tagging is required.