This phosphoramidite monomer is specifically utilized in the solid-phase synthesis of Morpholino oligonucleotides (PMOs) via the phosphoramidite method adapted for morpholine-based oligomers. Featuring a (2S,6R)-morpholine ring with N6-benzoyladenine at C6, N4-trityl protection, and a (dimethylphosphoramidochloridato)methyl group at C2, it enables the precise, stepwise addition of the adenine-morpholino unit to the growing PMO chain. Its high reactivity and stability ensure excellent coupling efficiency and fidelity in automated synthesizers, making it essential for producing high-quality PMOs used in antisense gene modulation, genetic research, developmental biology, diagnostics, and therapeutic applications like treatments for genetic disorders.