This compound is a protected cytidine phosphoramidite monomer primarily utilized in the synthesis of Morpholino oligonucleotides (PMOs), which are essential in genetic research and antisense therapeutic applications. It acts as a key reagent in the adapted phosphoramidite method, enabling the stepwise construction of Morpholino sequences. Its role is crucial in coupling morpholine units during the automated solid-phase synthesis process, ensuring high precision and efficiency. Additionally, it is employed in the development of antisense Morpholinos for gene silencing and targeted therapy for diseases such as Duchenne muscular dystrophy. The compound’s stability and reactivity make it a valuable tool in molecular biology and pharmaceutical research.