Used extensively in oligonucleotide synthesis, particularly in the production of DNA and RNA sequences. It serves as a key building block in solid-phase synthesis, enabling the precise addition of cytidine nucleotides to growing oligonucleotide chains. The protective groups, such as the tert-butyldimethylsilyl (TBDMS) and dimethoxytrityl (DMT), ensure selective reactivity and prevent unwanted side reactions during the synthesis process. Its phosphoramidite moiety facilitates efficient coupling, making it a critical reagent in the development of therapeutic oligonucleotides, research probes, and diagnostic tools. Commonly applied in fields like molecular biology, genetic research, and drug development for creating custom nucleic acid sequences.