3-Methylcytidine is primarily used in biochemical research, particularly in the study of RNA modifications and their functional roles. It serves as a critical tool for understanding the impact of methylation on RNA stability, structure, and interaction with proteins. This compound is also employed in the development of nucleoside analogs for potential therapeutic applications, such as antiviral or anticancer agents. Additionally, it aids in exploring epigenetic regulation and the mechanisms of gene expression at the RNA level. Researchers utilize 3-Methylcytidine to investigate its role in cellular processes and its potential as a biomarker for certain diseases.