1-Methylguanosine is primarily used in biochemical research to study RNA modifications and their impact on cellular processes. It serves as a key marker for understanding post-transcriptional changes in RNA, particularly in tRNA and rRNA, which influence gene expression and protein synthesis. Researchers utilize it to investigate its role in regulating RNA stability, translation efficiency, and cellular stress responses. Additionally, it is employed in studies related to cancer and neurodegenerative diseases, where abnormal RNA modifications are often observed. Its application extends to the development of diagnostic tools and therapeutic strategies targeting RNA modification pathways.