2'-Deoxy-1-methylguanosine is primarily utilized in biochemical and medical research, particularly in the study of DNA modifications and their biological implications. It serves as a key compound in understanding the role of methylated nucleosides in genetic regulation and cellular processes. Researchers employ it to investigate the effects of methylation on DNA structure, stability, and function, which is crucial for advancing knowledge in epigenetics. Additionally, it is used in the development of diagnostic tools and therapeutic strategies targeting methylation-related diseases, such as cancer. Its application extends to the synthesis of modified oligonucleotides for probing enzymatic activities and studying DNA repair mechanisms.