N2-Isobutyryl-2'-fluoro-2'-deoxyguanosine is primarily utilized in the field of nucleoside chemistry and pharmaceutical research. It serves as a key intermediate in the synthesis of modified nucleosides, which are essential for developing antiviral and anticancer drugs. The presence of the fluorine atom at the 2' position enhances the stability and binding affinity of the nucleoside to target enzymes, making it valuable for designing therapeutic agents. This compound is particularly significant in the study of RNA and DNA analogs, where it helps in understanding the mechanisms of viral replication and cellular processes. Additionally, it is employed in the development of prodrugs, improving the bioavailability and efficacy of nucleoside-based treatments. Its application extends to molecular biology, where it aids in the study of nucleic acid interactions and the design of novel therapeutic strategies.