N2-Phenylacetyl Guanosine is a protected form of guanosine commonly used as a building block in the chemical synthesis of RNA oligonucleotides. The phenylacetyl group serves as a protecting group for the N2 amino position of the guanine base, preventing unwanted side reactions during solid-phase synthesis. It is essential in biochemical research for producing modified RNA molecules to study nucleic acid structure, function, and modifications. This compound facilitates the exploration of gene expression, RNA-protein interactions, and RNA processing mechanisms. Additionally, it supports the development of therapeutic oligonucleotides targeting RNA-related diseases.