N6-(3-Methyl-2-butenyl)adenosine is primarily utilized in biochemical research due to its role as a modified nucleoside. It is often studied for its potential interactions with enzymes and receptors, particularly those involved in purine metabolism. This compound is also of interest in the development of novel therapeutics, as it may influence cellular signaling pathways. Additionally, it serves as a valuable tool in the synthesis of adenosine analogs, which are explored for their pharmacological properties, including anti-inflammatory and anti-cancer effects. Its unique structure makes it a candidate for probing biological mechanisms and designing targeted drug molecules.