α,β-Methyleneadenosine 5′-triphosphate lithium salt is widely utilized in biochemical research as a non-hydrolyzable ATP analog. It is particularly valuable in studying ATP-dependent enzymes and processes, as it binds to ATP-binding sites without being broken down, allowing researchers to investigate enzyme mechanisms and inhibition. This compound is also employed in structural studies, such as X-ray crystallography, to visualize enzyme-substrate interactions. Additionally, it is used in assays to measure kinase activity and other ATP-dependent reactions, providing insights into cellular signaling pathways and energy metabolism. Its stability and specificity make it a critical tool in understanding molecular and cellular functions.