Adenosine Deaminase (ADA) is widely used in clinical diagnostics to detect immunodeficiency disorders, particularly Severe Combined Immunodeficiency (SCID). It helps in assessing ADA deficiency, which leads to the accumulation of toxic metabolites, impairing immune function.

In biotechnology, ADA is utilized in enzyme replacement therapy to treat ADA-SCID patients. It converts toxic adenosine and deoxyadenosine into non-toxic inosine and deoxyinosine, restoring immune system functionality.

Additionally, ADA is employed in research to study purine metabolism and its role in cellular processes. It is also used in biochemical assays to measure adenosine levels, aiding in the study of metabolic pathways and drug development.

In agriculture, ADA is explored for its potential in enhancing plant resistance to pathogens by modulating adenosine-related signaling pathways. Overall, its applications span healthcare, research, and biotechnology.