Enpp-1-IN-1 is primarily used in research settings to study its inhibitory effects on the enzyme ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1). This enzyme plays a significant role in various biological processes, including nucleotide metabolism and immune regulation. By inhibiting ENPP1, Enpp-1-IN-1 helps researchers understand its function in diseases such as cancer, where ENPP1 is often overexpressed and contributes to tumor progression and immune evasion. Additionally, it is explored for its potential in modulating the immune response, particularly in enhancing the efficacy of cancer immunotherapies. Its application extends to studying metabolic disorders and vascular calcification, where ENPP1 activity is implicated.