Used in biochemical and molecular biology research as a nucleotide building block for DNA synthesis studies. Serves as a substrate in enzyme assays involving kinases, phosphorylases, and other nucleotide-metabolizing enzymes. Commonly employed in cell-free systems to investigate DNA replication and repair mechanisms. Also utilized in the development and optimization of diagnostic assays that require defined nucleotide components. Its disodium salt form enhances solubility and stability in aqueous reaction mixtures.