Used extensively in biochemical research, it plays a critical role in studying enzymatic reactions, particularly those involving dehydrogenases. It is essential in metabolic pathway analysis, helping to understand energy production and cellular respiration. Applied in redox reactions, it serves as a cofactor, facilitating electron transfer processes. Utilized in diagnostic assays, it aids in detecting various metabolic disorders. Additionally, it is employed in the development of biosensors for monitoring biochemical processes in real-time. Its role in NAD+/NADH cycling is pivotal in investigating cellular health and oxidative stress.