Used in biochemical research for RNA synthesis studies and as a substrate for enzymes like RNA polymerases and nucleotide-dependent enzymes. Commonly applied in molecular biology assays, including in vitro transcription, nucleotide labeling, and real-time monitoring of RNA production. Also utilized in cell culture media to support cell growth and metabolic studies, particularly in assays involving nucleotide metabolism. Its trisodium salt form enhances solubility and stability in aqueous solutions, making it suitable for enzymatic reactions and diagnostic applications.