Used primarily in the field of molecular biology, this modified nucleotide plays a crucial role in the synthesis of mRNA for therapeutic applications. It enhances the stability and translation efficiency of mRNA, making it particularly valuable in the development of mRNA vaccines and treatments. By incorporating it into mRNA, it helps reduce the immune system's recognition of the mRNA as foreign, thereby decreasing potential inflammatory responses. This modification is instrumental in advancing gene therapy and personalized medicine, enabling more effective and safer delivery of genetic information to target cells.